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Protocol Reference
Animal data only

TB-500 protocol

TB-500 (Thymosin Beta-4 fragment). The published work on this compound is animal or cell-culture research. No human dosing schedule exists to cite.

Category
Healing & Repair
Common vials
10 mg
Half-life
Not established for the fragment
Route in the literature
Subcutaneous or intramuscular in animal work
Evidence level
Animal data only

How TB-500 works

A synthetic fragment of thymosin beta-4, the actin-sequestering protein. The parent protein has been studied in human trials for wound healing and dry eye; TB-500 as sold is the fragment, not the full protein.

TB-500 dosing — what the literature actually supports

Animal data only. The human trials in this area studied full-length thymosin beta-4, not the TB-500 fragment sold as a research chemical, and mostly used topical or ophthalmic administration rather than injection. There is no published human trial of injected TB-500 establishing a schedule. Treat the two as different things — a citation for thymosin beta-4 is not a citation for TB-500.

We publish a schedule when there is a citable human source and we say so when there is not. Filling this space with a number nobody measured would make the page look more complete and be worth less than nothing to you.

Reconstitution and measurement

This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for TB-500: 10 mg.

Open the TB-500 calculator → Unit converter

Storage and stability

Dry powder
Cold, dark and dry. Lyophilized peptide is the stable form — keep it that way until you intend to use it.
After reconstitution
Refrigerate at 2–8 °C, protect from light, and plan around a limited window. Do not freeze a reconstituted vial — freeze-thaw damages peptides.
Mixing
Run bacteriostatic water down the inside wall of the vial rather than onto the powder, then swirl. Never shake.
Inspect before use
A clear solution is expected. Cloudiness or particles mean discard, not use.

Full storage guide → · Cloudy solution?

Reported effects

  • No human safety data for the injected fragment.
  • Full-length thymosin beta-4 was well tolerated in the trials that studied it, which does not transfer to the fragment by a different route.

Sourcing TB-500

Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.

TB-500 at Summit Research Supply → Grade a COA first

Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.

For laboratory research use only. This page documents what the published literature reports about TB-500. It is not a recommendation, not a treatment plan, and not medical advice. Compounds referenced are sold strictly as research chemicals and are not for human or veterinary use. Some supplier links are affiliate links and may earn us a commission. This never affects tier placement or review conclusions.
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