SS-31 (Elamipretide) Reconstitution & Dosage Calculator
This free SS-31 calculator turns your vial size, the bacteriostatic water you add, and your target amount into a concentration, a draw volume, and the exact units on a U-100 insulin syringe. SS-31 — also known as elamipretide — is a mitochondria-targeted tetrapeptide that concentrates at the inner mitochondrial membrane, and unlike most peptides here it is studied in whole-milligram amounts. This is a measurement tool — it does not recommend an amount.
SS-31 reconstitution calculator
Syringe
U-100 insulinPeptide in vial
Target amount
Bacteriostatic water
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Quick summary
- Converts vial size (mg), bacteriostatic water (mL), and a milligram amount into concentration, draw volume, and U-100 units.
- Reference math for the 10 mg vial SS-31 is supplied in, at milligram rather than microgram scale.
- Educational research and measurement tool only — it does not diagnose, treat, or recommend an amount.
What this SS-31 calculator does
This calculator does one job well: it turns your vial size, the amount of bacteriostatic water you add, and your target amount into a concentration (mg/mL), a draw volume (mL), and the matching units on a U-100 insulin syringe. Change any input and the result updates instantly.
SS-31 ships as a freeze-dried powder. Before it can be measured into a syringe it has to be reconstituted — dissolved in bacteriostatic water. How much water you add sets the concentration, and the concentration sets how many units each amount works out to. The presets above cover the most common SS-31 vial setups; use the custom fields for anything else.
How to use the SS-31 calculator
Pick your syringe
Choose the U-100 insulin syringe you'll draw with. Smaller syringes (0.3 mL / 30u) have finer lines, which helps when the draw is small.
Enter your vial and water
Set the milligrams in your SS-31 vial and the bacteriostatic water you added. Together these set the concentration.
Set your target amount
Toggle mg or mcg and pick (or type) the amount you're measuring for. The calculator does the conversion for you.
Read the draw
The result panel shows concentration, draw volume, and the exact U-100 units to pull, plus how many doses your vial contains.
SS-31 reconstitution math, explained
The math is short. Concentration = vial size ÷ bacteriostatic water. Draw volume = target amount ÷ concentration. Units = draw volume × 100 (a U-100 syringe reads 100 units per mL). The table below shows common SS-31 setups and the units for a 1 mg amount at each.
| Vial size | Bac water | Concentration | Units per 1 mg |
|---|---|---|---|
| 10 mg | 1.0 mL | 10 mg/mL | 10 units |
| 10 mg | 2.0 mL | 5 mg/mL | 20 units |
| 10 mg | 2.5 mL | 4 mg/mL | 25 units |
| 10 mg | 5.0 mL | 2 mg/mL | 50 units |
| 50 mg | 5.0 mL | 10 mg/mL | 10 units |
SS-31 amount-to-units reference
How common amounts convert to U-100 syringe units at two example concentrations. These are arithmetic conversions for reference, not a recommendation of any amount.
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 1 mg (1000 mcg) | 0.2 mL | 20 units |
| 2.5 mg (2500 mcg) | 0.5 mL | 50 units |
| 5 mg (5000 mcg) | 1 mL | 100 units |
| 10 mg (10000 mcg) | 2 mL | 200 units |
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 1 mg (1000 mcg) | 0.1 mL | 10 units |
| 2.5 mg (2500 mcg) | 0.25 mL | 25 units |
| 5 mg (5000 mcg) | 0.5 mL | 50 units |
| 10 mg (10000 mcg) | 1 mL | 100 units |
Mixing, color & storage tips
Milligram scale means large draws
SS-31 amounts are whole milligrams, not micrograms, so draws are much larger than for a typical peptide. At 5 mg/mL a 5 mg target is a full 1 mL — the entire capacity of a U-100 syringe. A more concentrated mix keeps larger amounts inside one syringe.
Mixing without foam
Add the bacteriostatic water slowly against the inside wall of the vial and swirl gently until fully dissolved. Do not shake.
Storage
Keep the dry powder cold and dark. Refrigerate once reconstituted, keep it out of light, and plan around a limited usable window. Do not freeze a mixed vial.
SS-31 vs other mitochondrial compounds
SS-31 targets the inner mitochondrial membrane directly, which is a different mechanism from MOTS-c (a mitochondrial-derived signalling peptide) or NAD+ (a cofactor). They are often discussed together but they are not substitutes for one another.
SS-31 supplies checklist
A simple reconstitution shopping list. Confirm vial size and batch documentation before you buy.
SS-31
- Batch COA on every vial
- Third-party purity tested
- U.S. fulfillment, discreet shipping
SS-31 — frequently asked questions
What does this SS-31 calculator tell me?
It converts your vial size, bacteriostatic water volume, and target amount into concentration in mg/mL, draw volume in mL, and the matching units on a U-100 insulin syringe. It is a measurement tool, not a recommendation.
Is the SS-31 calculator free?
Yes — free, browser-based, and no signup is required.
Is SS-31 the same as elamipretide?
Yes. SS-31 is the research designation and elamipretide is the international nonproprietary name for the same mitochondria-targeted tetrapeptide. You will see both used in the literature.
How much bacteriostatic water do I add to a 10 mg SS-31 vial?
A 10 mg vial with 2 mL makes 5 mg/mL, so a 2.5 mg target is 50 units. Using 1 mL makes 10 mg/mL and halves that to 25 units, which matters because SS-31 amounts are large enough to approach a full syringe.
How many units is 2.5 mg of SS-31?
At 5 mg/mL it is 50 units (0.5 mL). At 10 mg/mL it is 25 units (0.25 mL). Units always depend on your exact concentration.
How long does a reconstituted SS-31 vial last?
Refrigerate it, keep it dark, and plan around a limited usable window. Do not freeze a mixed vial; keep the dry powder cold for longer storage.
Is this medical advice?
No. This page and calculator are for education and research planning only. Products referenced are sold strictly as research chemicals and are not for human or veterinary use.
Sources & further reading
Related calculators
SS-31 protocol & evidence
Dosing for SS-31 comes from published human trials. The protocol page carries the schedule that was actually administered, with the citation.
