AOD-9604 Reconstitution & Dosage Calculator
This free AOD-9604 calculator turns your vial size, the bacteriostatic water you add, and your target amount into a concentration, a draw volume, and the exact units on a U-100 insulin syringe. AOD-9604 is the C-terminal 176-191 fragment of human growth hormone, studied for metabolic rather than growth-promoting activity, and it is handled in microgram amounts. This is a measurement tool — it does not recommend an amount.
AOD-9604 reconstitution calculator
Syringe
U-100 insulinPeptide in vial
Target amount
Bacteriostatic water
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Quick summary
- Converts vial size (mg), bacteriostatic water (mL), and a microgram amount into concentration, draw volume, and U-100 units.
- Reference math for the 5 mg vial AOD-9604 is supplied in.
- Educational research and measurement tool only — it does not diagnose, treat, or recommend an amount.
What this AOD-9604 calculator does
This calculator does one job well: it turns your vial size, the amount of bacteriostatic water you add, and your target amount into a concentration (mg/mL), a draw volume (mL), and the matching units on a U-100 insulin syringe. Change any input and the result updates instantly.
AOD-9604 ships as a freeze-dried powder. Before it can be measured into a syringe it has to be reconstituted — dissolved in bacteriostatic water. How much water you add sets the concentration, and the concentration sets how many units each amount works out to. The presets above cover the most common AOD-9604 vial setups; use the custom fields for anything else.
How to use the AOD-9604 calculator
Pick your syringe
Choose the U-100 insulin syringe you'll draw with. Smaller syringes (0.3 mL / 30u) have finer lines, which helps when the draw is small.
Enter your vial and water
Set the milligrams in your AOD-9604 vial and the bacteriostatic water you added. Together these set the concentration.
Set your target amount
Toggle mg or mcg and pick (or type) the amount you're measuring for. The calculator does the conversion for you.
Read the draw
The result panel shows concentration, draw volume, and the exact U-100 units to pull, plus how many doses your vial contains.
AOD-9604 reconstitution math, explained
The math is short. Concentration = vial size ÷ bacteriostatic water. Draw volume = target amount ÷ concentration. Units = draw volume × 100 (a U-100 syringe reads 100 units per mL). The table below shows common AOD-9604 setups and the units for a 1 mg amount at each.
| Vial size | Bac water | Concentration | Units per 1 mg |
|---|---|---|---|
| 5 mg | 1.0 mL | 5 mg/mL | 20 units |
| 5 mg | 2.0 mL | 2.5 mg/mL | 40 units |
| 5 mg | 2.5 mL | 2 mg/mL | 50 units |
| 5 mg | 3.0 mL | 1.67 mg/mL | 60 units |
| 10 mg | 2.0 mL | 5 mg/mL | 20 units |
AOD-9604 amount-to-units reference
How common amounts convert to U-100 syringe units at two example concentrations. These are arithmetic conversions for reference, not a recommendation of any amount.
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 250 mcg (0.25 mg) | 0.1 mL | 10 units |
| 300 mcg (0.3 mg) | 0.12 mL | 12 units |
| 500 mcg (0.5 mg) | 0.2 mL | 20 units |
| 1000 mcg (1 mg) | 0.4 mL | 40 units |
| Amount | Volume (mL) | U-100 units |
|---|---|---|
| 250 mcg (0.25 mg) | 0.05 mL | 5 units |
| 300 mcg (0.3 mg) | 0.06 mL | 6 units |
| 500 mcg (0.5 mg) | 0.1 mL | 10 units |
| 1000 mcg (1 mg) | 0.2 mL | 20 units |
Mixing, color & storage tips
A dilute mix reads better
At 2.5 mg/mL a 300 mcg target is 12 units, which sits comfortably in the readable middle of a U-100 syringe. The same amount at 5 mg/mL is only 6 units, where a small misread is proportionally a much larger error.
Mixing without foam
Add the bacteriostatic water slowly against the inside wall of the vial and swirl gently until clear. Do not shake — this is a peptide fragment and agitation can degrade it.
Storage
Keep the dry powder cold and dark. Refrigerate once reconstituted, keep it out of light, and plan around a limited usable window. Do not freeze a mixed vial.
Not growth hormone
AOD-9604 is a fragment of the hGH molecule, not hGH itself, and the literature separates its metabolic activity from the growth-promoting activity of the full protein. If you are comparing it against GH-axis secretagogues, they are a different mechanism class entirely.
AOD-9604 supplies checklist
A simple reconstitution shopping list. Confirm vial size and batch documentation before you buy.
AOD-9604
- Batch COA on every vial
- Third-party purity tested
- U.S. fulfillment, discreet shipping
AOD-9604 — frequently asked questions
What does this AOD-9604 calculator tell me?
It converts your vial size, bacteriostatic water volume, and target amount into concentration in mg/mL, draw volume in mL, and the matching units on a U-100 insulin syringe. It is a measurement tool, not a recommendation.
Is the AOD-9604 calculator free?
Yes — free, browser-based, and no account is required.
How much bacteriostatic water do I add to a 5 mg AOD-9604 vial?
A 5 mg vial with 2 mL makes 2.5 mg/mL, which turns a 300 mcg target into a 12-unit draw. Using 1 mL makes 5 mg/mL and halves the units to 6. The dilute mix is the easier one to measure accurately.
How many units is 300 mcg of AOD-9604?
At 2.5 mg/mL it is 12 units (0.12 mL). At 5 mg/mL it is 6 units (0.06 mL). Units always depend on your exact concentration.
Is AOD-9604 the same as HGH?
No. It is the 176-191 C-terminal fragment of the human growth hormone molecule, not the full protein, and it is studied for different activity. They are not interchangeable.
How long does a reconstituted AOD-9604 vial last?
Refrigerate it, keep it dark, and plan around a limited usable window. Do not freeze a mixed vial; keep the dry powder cold for longer storage.
Is this medical advice?
No. This page and calculator are for education and research planning only. Products referenced are sold strictly as research chemicals and are not for human or veterinary use.
Sources & further reading
Related calculators
AOD-9604 protocol & evidence
Dosing for AOD-9604 comes from published human trials. The protocol page carries the schedule that was actually administered, with the citation.